Lung collagen heterogeneity. Synthesis of type I and type III collagen by rabbit and human lung cells in culture.
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منابع مشابه
Lung collagen heterogeneity. Synthesis of type I and type III collagen by rabbit and human lung cells in culture.
The fetal and adult lung have a constant level of collagen synthesis that represents 4-5% of the total amino acids incorporated into lung protein. Prior studies have demonstrated that this collagen is not homogeneous but rather is composed of at least two collagen types, I and II, each localized to specific lung structures. Although it is known that explants of rabit lung parenchyma and blood v...
متن کاملType I and type III collagen mRNA expression in human lung fibroblasts.
The predominant histological features of pulmonary fibrosis are an accumulation of collagen in the alveolar interstitium. and an increase in the number of fibroblasts [ l ] . There is an alteration in collagen production with an increase in Type I collagen relative to Type II. The mechanisms involved in this regulation of collagen production at transcriptional and translational level are poorly...
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Whole neonate rabbit corneas and adult corneas containing 2-week-old scars were incubated in the presence of [14C] glycine. Radiolabeled collagen extracted from the corneas and scar tissue were analyzed by sodium dodecylsulfate/polyacrylamide gel electrophoresis and fluorography to determine the types and relative quantity of collagen polypeptides present and synthesized by these tissues. In ad...
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A fluorescence-based method using the cell sorter has been devised to separate rat lung fibroblasts into subpopulations. Type I or type III collagen antiserum was used as the primary antibody to react with parent rat lung fibroblasts. This was followed by a fluorescein-conjugated secondary antibody. Specificity of the primary collagen antibody was determined using a monoclonal beta-actin antibo...
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Asthma is characterized by chronic inflammation of the airway wall with the presence of activated T helper 2 (Th2) lymphocytes. The current study assessed the ability of Th2 cytokines to modulate fibroblast-mediated contraction of collagen gels to determine if Th2 cytokines could contribute to tissue remodeling by altering mesenchymal cell contraction. Human fetal lung fibroblasts, human adult ...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1976
ISSN: 0021-9738
DOI: 10.1172/jci108250